The adenovirus isolate, obtained from a fecal sample in Mumbai and maintained at ICMR‑NIV, is identified as Human Adenovirus type 1 (HAdV‑1), a serotype within the Mastadenovirus genus of the Adenoviridae family. It is chiefly linked to respiratory infections in children, with occasional cases of conjunctivitis and gastrointestinal illness, and its characterization is confirmed through RT‑PCR and whole‑genome sequencing.
The Crimean-Congo Haemorrhagic Fever virus isolate, sourced from a clinical serum sample collected in Gujarat, is maintained at ICMR-NIV. Molecular confirmation through RT-PCR and full-genome sequencing supports accurate characterization. This isolate can enable research on disease mechanisms, antiviral assessment, diagnostic refinement, and preparedness efforts for regions experiencing recurring CCHF activity.
The Chandipura virus isolate, obtained from clinical material collected from Gujrat in India and preserved at ICMR-NIV. It has been characterized through genome sequencing and molecular assays targeting a conserved viral gene. As part of the national viral collection, it supports studies on neurotropic viral infections, diagnostic development, vector-borne disease surveillance, and preparedness programs.
Enterovirus A includes Coxsackievirus isolates, derived from clinical samples collected in Pune and preserved at ICMR NIV, include four distinct serotypes: A6, A16, A24, and B4. Each serotype represents a genetically unique strain with characteristic disease associations A6 with Hand, Foot, and Mouth Disease (HFMD) marked by severe rashes, A16 with mild HFMD sometimes linked to meningitis, A24 with acute hemorrhagic conjunctivitis outbreaks, and B4 with myocarditis, pancreatitis, Type 1 diabetes, and systemic illness. Their identity and diversity have been confirmed through genome sequencing and molecular assays targeting conserved viral genes.
The Enterovirus B isolates, derived from clinical samples collected in Pune and preserved at ICMR NIV, include two distinct serotypes 29 and 30. Their identity and diversity have been confirmed through genome sequencing and molecular assays targeting conserved viral genes.
The Enterovirus 71 isolates, derived from clinical samples collected from Cerebrospinal fluid (CSF) of infected patients from Jaunpur, Uttar Pradesh and Faridabad, Haryana preserved at ICMR NIV. Isolate was confirmed by RT-PCR and WGS. The Sub-genotypes available are sub-genotype-C1 and sub-genotype-D.
The Influenza A (H1N1 pdm09) isolate collected in Bangalore is maintained at ICMR-NIV. Characterization includes full-genome sequencing and real-time PCR confirmation. This isolate supports genomic surveillance, resistance profiling, and refinement of national vaccine-strain selection strategies for seasonal influenza.
The Influenza A H3N2 isolate from Puducherry is preserved at ICMR-NIV and confirmed through genome sequencing and real-time PCR. As part of national influenza monitoring, the isolate may be helpful in antigenic analysis, antiviral-resistance tracking, and seasonal vaccine-strain recommendations.
The Influenza B Victoria-lineage isolate obtained in Pune is preserved at ICMR-NIV. Characterization includes full-genome sequencing and real-time PCR confirmation. The isolate can contribute to national influenza surveillance by supporting strain monitoring, antigenic evaluation, and vaccine-formulation development.
The Kyasanur Forest Disease (KFD) virus isolate, obtained from Shivamogga, Karnataka, India, is preserved at ICMR National Institute of Virology (NIV), Pune. Characterization includes full genome sequencing and real time PCR confirmation. The isolate can contribute to research on viral pathogenesis, diagnostic assay development, vaccine studies, and public health preparedness against tick borne viral outbreaks.
The measles virus isolate, collected from a throat swab in Gujarat, is maintained in the national reference collection at ICMR-NIV. Characterization includes genome-based typing and standard molecular assays focused on key viral genes. This isolate can support measles elimination efforts through molecular surveillance, vaccine monitoring, and understanding lineage circulation.
The Mpox virus isolate has been obtained from Kollam, Kerala and is preserved at ICMR National Institute of Virology (NIV), Pune. Characterization includes full genome sequencing and real time PCR confirmation. The isolate can contribute to diagnostic assay development, antiviral and vaccine research, and strengthening public health preparedness against Mpox outbreaks.
The mumps virus isolate, obtained from an oral swab in Maharashtra, is preserved at ICMR-NIV. It is characterized through genetic analysis and RT-PCR targeting a routinely assessed viral region. This isolate can serve as a valuable tool for mumps surveillance efforts, supporting outbreak investigation, lineage mapping, vaccine-effectiveness assessments, and diagnostic improvements.
The Nipah virus isolate, obtained from human oropharyngeal samples during an outbreak in Kerala, is preserved at ICMR-NIV. Characterization includes complete genome sequencing and RT-PCR using Nipah-specific targets. This isolate can aid studies on pathogen evolution, diagnostic strengthening, antiviral research, and outbreak preparedness, given Nipah's high public-health relevance.
The Rhino virus isolate, obtained from Idukki, Kerala, India, is preserved at ICMR‑National Institute of Virology (NIV), Pune. It was obtained from nasopharyngeal swab (NPS) samples of humans. The isolate can contribute to respiratory virus research, diagnostic assay validation, antiviral screening, and public health preparedness for common cold and related respiratory infections.
The rubella virus isolate, collected in the early years of national surveillance from a suspected case in Maharashtra, is maintained at ICMR-NIV. As a historic genotype reference with a detailed sequencing record, it is vital for understanding rubella evolution, supporting congenital rubella syndrome tracking, evaluating vaccine performance, and strengthening national elimination initiatives.
The SARS-CoV-2 virus variants (Beta Coronavirus, Delta Variant, Omicron), originating from a clinical respiratory specimen collected in India, is preserved at ICMR-NIV, Pune and ICMR - NIRBI. It is characterized through real-time RT-PCR targeting a conserved viral gene, ensuring accurate identification. As part of the national repository, this isolate can support research on diagnostics, therapeutic evaluation, vaccine-related studies, and preparedness initiatives.
The Zika virus isolate was obtained from Pune, Maharashtra, India, is preserved at ICMR‑National Institute of Virology (NIV), Pune. The isolate can contribute to diagnostic assay development, vaccine and antiviral research, molecular epidemiology, and public health preparedness against Zika virus outbreaks, alongside additional reference isolates from Jaipur, Rajasthan (human serum) and Uganda (Rhesus monkey serum) that strengthen comparative genomic studies and evolutionary research.
